Code

Github : https://github.com/Cloud-Lab-Commons/ecl
All code is shared freely under the Apache 2.0 license. https://opensource.org/license/apache-2-0/

Or view the code online!

Code in the sandbox section has not been fully debugged.

Sandbox – Pages

Singleplex qPCR and melt curve v 1.0
https://www.emeraldcloudlab.com/documentation/publish/notebookPage?id=id:n0k9mGkL4jrW

This code is meant to run a singleplex qPCR in triplicate with a melt curve requiring only forward primer, reverse primer, and template objects as input. Six reactions are automatically run (3 with your DNA template and 3 no template controls using buffer)

Sandbox – Scripts

Agarose gel
https://www.emeraldcloudlab.com/documentation/publish/notebookPage?id=id:jLq9jXqwNEVa

Agarose gels can be finicky at low volumes. This provides an advised set of volumes that run robustly

Sandbox – Functions

Helper functions
https://www.emeraldcloudlab.com/documentation/publish/notebookPage?id=id:AEqRl9qmBLzw

Functions to include in all scripts. Includes Gantt chart