Code
Github : https://github.com/Cloud-Lab-Commons/ecl
All code is shared freely under the Apache 2.0 license. https://opensource.org/license/apache-2-0/
Or view the code online!
Code in the sandbox section has not been fully debugged.
Sandbox – Pages
Singleplex qPCR and melt curve v 1.0 https://www.emeraldcloudlab.com/documentation/publish/notebookPage?id=id:n0k9mGkL4jrW
This code is meant to run a singleplex qPCR in triplicate with a melt curve requiring only forward primer, reverse primer, and template objects as input. Six reactions are automatically run (3 with your DNA template and 3 no template controls using buffer)
Sandbox – Scripts
Agarose gel https://www.emeraldcloudlab.com/documentation/publish/notebookPage?id=id:jLq9jXqwNEVa
Agarose gels can be finicky at low volumes. This provides an advised set of volumes that run robustly
Sandbox – Functions
Helper functions https://www.emeraldcloudlab.com/documentation/publish/notebookPage?id=id:AEqRl9qmBLzw
Functions to include in all scripts. Includes Gantt chart